Sunderland Women drew 2-2 and lost out on penalties against Durham in the second Continental Tyres League Cup group stage match.
Mollie Lambert gave Durham the lead in the fifth minute, with Natasha Fenton and Brianna Westrup scoring in the 15th and 42nd to give the Lasses the lead going into the second half.
Saoirse Noonan scored a last-minute equaliser, with the visitors winning 3-1 on penalties.
Sunderland boss Mel Reay made seven changes from the side that won at Maiden Castle on Sunday, with seven teenagers also in the squad.
Lambert gave Durham the lead early on, firing a right-footed shot into the far corner.
The Black Cats responded well and 10 minutes later, Emily Scarr whipped a corner to the back post, with Fenton heading back across and into the back of the net to equalise.
Sunderland controlled the game well for large parts of the first half, keeping possession and looking threatening on the counter.
The Lasses made use of another set piece for the second goal, this time Fenton assisting with a free-kick for Westrup to head home.
Katy Watson was unlucky to have a goal ruled out, getting in behind, looking to have kept her line and poking the ball into the back of the net, but the offside was called.
A scramble in the box eventually fell to Noonan in the dying seconds of the game, and the forward fired past Megan Borthwick, forcing the game to a penalty shootout.
Durham scored three and Sunderland only managed one penalty goal, meaning the away side claimed the bonus point in group B.
The Lasses now turn their attentions back to the league as they host Blackburn at Eppleton on Sunday.
SAFC Women: Borthwick, Brown, McCatty, Westrup © (West 46’), Ede (Beer 46’), Fenton (Kitching 46’), McInnes, Rouse (Jones 76’), Corbyn, Scarr, Watson.
Subs not used: Moan, Ejupi, Griffiths, McAteer, Parker.